The interference of different Ganoderma lucidum in colorectal cancer cell(HT-29)

• Main Authors: SHIUN,SHENG-JIE, 孫聖傑
• Other Authors: WANG,HAI-LUNG
• Format: Others
• Language: zh-TW
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/54951816570198198554

碩士 === 元培醫事科技大學 === 醫學檢驗生物技術系碩士班 === 105 === The interference of different Ganoderma lucidum in colorectal cancer cell(HT-29) Student: Sheng-Chieh Sun Adviser:. Hai-Long Wang Graduate Institute of Medical Laboratory Science and Biotechnology, Yuanpei University of Medical Technology Abstract Ganoderma lucidum is a type of edible herbs that has been used as natural health-promoting food in Asia for a long time, with several pharmacological features. Many researches have proven that Ganoderma lucidum is nontoxic and can regulate the immunological functions, inhibit tumor growth, increase resistance to oxidation, protect the liver, decrease blood glucose and combat allergies. This study aimed to analyze the biological activities of Ganoderma strain fermented products derived from wild Ganoderma lucidum strains collected in the Xinpu region of Hsinchu County, and code named Ganoderma No. 1, Ganoderma No. 2, Ganoderma A, Ganoderma B, Ganoderma N, Ganoderma Xinpu and Standard Ganoderma CCRC36021. In this study, the seven types of Ganoderma lucidum strains were respectively subject to liquid culture for 28 days. Molecular sieves were used to separate the culture solution of different molecular weights (those greater than 100kDa, between 30kDa and 100kDa, and those smaller than 30kDa), and then different concentrations of the culture solution were added to the colon cancer cells (HT-29) for culturing. MTT assay was adopted to determine the cell survival rate, while a flow cytometer was used to observe the cell cycle. The results showed that Standard Ganoderma CCRC36021 exhibited the most significant inhibition of HT-29 colon cancer cell growth in the 100kDa+ group versus Control comparison; while Ganoderma N and Standard Ganoderma CCRC36021 had the most significant inhibition of HT-29 colon cancer cell growth compared to the other strains in the 30kDa to 100kDa group versus Control comparison. In the cell cycle experiment involving the culture solution of Ganoderma N and Standard Ganoderma CCRC36021 on HT-29 colon cancer cells, the results showed that the culture solution of Ganoderma N with molecular weight greater than 100kDa and that between 30kDa and 100kDa significantly retarded the growth of HT-29 colon cancer cell strains in G1 phase, compared to the Control group. The culture solution of Standard Ganoderma CCRC36021 with molecular weight greater than 100kDa showed the effect of increasing the cell cycle of HT-29 colon cancer cell strains in G1 phase, while the culture solution with molecular weight between 30kDa and 100kDa significantly retarded the growth of HT-29 colon cancer cell strains in the G1 phase, compared to the Control group. This study shows that the polysaccharides of wild Ganoderma N strains collected in Xinpu Township, Hsinchu County, have better inhibition performance in relation to the growth of cancer cells and obviously retarded the growth of HT-29 colon cancer cell strains in the G1 phase. Keywords: Ganoderma lucidum, polysaccharides