The Distribution of Mycoplasma Species in Orals of Young Adults in Taiwan – by Nucleic Acid Testing

• Main Authors: Hsueh, Han-Hung, 薛翰鴻
• Other Authors: Kung, Chien-Min
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/3qn7b5

碩士 === 元培醫事科技大學 === 醫學檢驗生物技術系碩士班 === 105 === Backgrounds: Mycoplasma, size of 0.1-0.25µm, is the minimum prokaryotic microorganism which belongs to the Mycoplasmataceae and has the capacity of self-replication in artificial culture medium. Different from other bacteria, the mycoplasma has none cell walls. Therefore, many antibiotics, such us Penicillin or β-lactam drugs that inhibits cell wall synthesis are invalid for the mycoplasmas. Mycoplasma is a common opportunistic microorganism in the mammalia, reptilia, fish, birds and plants. Generally, there are eight mycoplasma species in human, including Mycoplasma hominis, Mycoplasma fermentans, Mycoplasma pneumoniae, Mycoplasma salivarium, Mycoplasma orale, Mycoplasma buccale, Mycoplasma faucium and Mycoplasma genitalium. Human oral is the most organ for mycoplasma gathering, as well as infectious pathogens that awaiting opportunities all the time. So far, there is no report concerning about the distribution of mycoplasma species in human orals, especial for young adults in Taiwan. Therefore, this study intends to evaluate the distribution of mycoplasma species in the orals of young adults in Taiwan. Methods: Ninety-six subjects(age,18-22) were recruited. All oral samples were collected by sterile cotton swabs with the standard collection manuals, and analyzed by the nucleic acid testing. The universal mycoplasma primer and the other five specificity mycoplasma primers were used to identify Mycoplasma hominis, Mycoplasma fermentans, Mycoplasma pneumoniae, Mycoplasma salivarium and Mycoplasma oraleby polymerase chain reactions. Results: The results showed that 56 subjects of all (58.3%) specimens presented positive reactions by PCR using the universal mycoplasma primers. Among those positive subjects, 42.71% were female subjects, while 15.63% were male subjects. Further testing, five specific mycoplasma primer PCR were testing for 56 positive subjects. The results had shown that 37 subjects (66.07%) were positive reaction against the Mycoplasma salivarium (62.1% for females subjects and 53.57% for males subjects), 30 subjects (53.57%) presented positive reaction against the Mycoplasma orale (80% for females subjects and 20% for males subjects), and 4 subjects (8.3%) presented positive reaction against the Mycoplasma pneumoniae(100% for females). Moreover, according living areas of subjects, this study also found that the distribution of Mycoplasma salivarium (54.05%) and Mycoplasma orale (76.67%) were higher in the northern areas than in other areas of Taiwan. However, whether the incidences of diseases is relative with the results of this study, it needs further investigation.