Rosmarinic acid alleviated selenite-induced cataract in rats

• Main Authors: Wu, Jia-Ying, 吳佳螢
• Other Authors: Tsai, Chia-Fang
• Format: Others
• Language: zh-TW
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/493n48

碩士 === 環球科技大學 === 生物技術研究所 === 105 === Cataracts are the most common cause of human blindness worldwide, and their incidence is likely to increase as the environmental variation. Cataract is caused by mutation of lens-specific proteins which, the infection, the flesh wound or other diseases cause, can let the crystalline lens of the lens be muddy. In the present study, the aim was to evaluate the protection effects on sodium selenite-induced cataractogenesis. The rosemary acid (Rosmarinic acid, is called RA) is one kind of water-soluble natural phenol acids compound which the separation obtains from the orthodon plant rosemary, has the strong oxidation resistance activeness, conducives toward favor prevented the free radical creates cell damage. Cataract formation is because excessively many free radicals and active oxygen (ROS) produce the oxidized pressure, then cataract production. This research investigated natural oxidation inhibitor, rosemary acid, to prevent selenite-induced cataracts in a rat model. SD rat pups were divided into five experimental groups. Normal group (Contorl) ,Selenite -challenged group, RA -treated (5, 10, 50 mg/kg) group. Animals in the control group and selenite-challenged group were injected with vehicle alone, while those in RA –treated group received a intraperitoneal, injection of RA on days 0-6. Sodium selenite (2.46 mg/kg body weightgiven 4h after the RA injection on day1) was administered to rats inI selenite-challenged group and RA-treated group to induce cataract formation. Lenses were observed with dissecting microscopy, and lens-specified protein, inflammatory protein and antioxidant activity in the lens were measured on day 7. Our data suggest that the selenite-challenged group after the injected subcutaneously fourth date can be observed by the visual obvious cataract formation, but the RA-treated group according to the intraperitoneal injections rosemary acid concentration promotion, cataract forms slightly. The lens-specific proteins such as filensin and calpain is associated with the transparency of the lens. Treatment with rosemary acid could increased the expression of filensin, calpain, Nrf-2, SOD, GSH-Rd, GSH-Px when compared with the selenite-challenged group. Treatment with rosemary acid could decreased the expression of COX-2, NF-κ B, iNOS, caspase 8, caspase 3, TBARS when compared with the selenite-challenged group. Len hostopathology also showed that rosemary acid reduced the incidence of len lesion induced by sodium selenite.The results suggest that rosemary acid exhibits potent len-protective effects.