| Summary: | 碩士 === 大同大學 === 生物工程學系(所) === 105 === After different codon optimization, the corresponding gene for YS, a human low-molecular-weight protein, was artificially synthesized to generate two genes, ys1 and ys2, which were subsequently constructed onto E. coli plasmids, pET22b, pET23a, pET23am (modified pET23a) and pET24a, followed by transformation into E. coli BL21 (DE3) for YS expression. Part of the 5’terminal DNA sequences of ys1 and ys2 were modified to decrease the G+C content, giving rise to ays1, bys1, cys1, ays2, bys2 and cys2 which were then ligated with pET23a to generate recombinant plasmids, pAYS1, pBYS1, pCYS1, pAYS2, pBYS2 and pCYS2, respectively, because ys1constructed onto pET23a gave better YS expression. All recombinant plasmids were also transformed into E. coli BL21 (DE3). After induction with IPTG, E. coli BL21 (DE3) carrying pBYS1 exhibited the best YS expression. Nevertheless, pBYS2 derived from ys2 subject to modification of the 5’terminal DNA sequences also had significant YS expression contrary to original ys2 constructed onto pET23a showing little expression.
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