Competition of Tet-off system enhances Biological Noise in Saccharomyces cerevisiae

• Main Authors: Chi-Fu Chen, 陳吉甫
• Other Authors: Che-Chi Shu
• Format: Others
• Language: zh-TW
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/uumw8w

碩士 === 國立臺北科技大學 === 化學工程與生物科技系化學工程碩士班（碩士在職專班） === 105 === The phenotypic characteristics of biological individuals are the result of a series of gene expression and protein-protein interactions, such as the process of signaling. But if there is noise involved in which will cause individual differences even though the beginning of the signal is the same, called stochastic processes. Randomness affects gene expression and behavior, which plays an important role in the organism. We used the Saccharomyces cerevisiae experiment to demonstrate manipulate the intensity of the internal noise and utilize it to prompt the population to the desired mode from a single gene. We constructed two Tet-off systems with different colors of the fluorescent protein gene; green fluorescent protein and red fluorescent protein gene, and placed in the same regulatory mechanism and control added quantitative doxycycline inducer, and then observed that both produced a competitive response. When Tet-off system is stabilize controlled by an inducer, however other Tet-off system competitors at the moment, it is possible to allow the population to desired mode and the intensity and distribution of the two fluorescent proteins were analyzed by flow cytometry to assess the interference degree of the noise. Our observed data to calculation have the negative correlation effect, and then we understand the molecular mechanism of biological noise. It can be seen that biological noise will affect the choice of cell fate, so that cells have different roles between individuals.