Quantitative concentration measurement of Epigallocatechin gallate by high performance liquid chromatography

• Main Authors: Lun-Hao Hsu, 許倫豪
• Other Authors: Hsu-Wei Fang Ph.D.
• Format: Others
• Language: zh-TW
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/j86976

碩士 === 國立臺北科技大學 === 化學工程與生物科技系化學工程碩士班（碩士在職專班） === 105 === Epigallocatechin gallate (EGCG) is a beneficial component for people, many studies have pointed out that EGCG antioxidant effect is very good, long-term consumption can lower blood pressure, reduce the incidence of cardiovascular disease. EGCG has recently been used in skin care products, so that products can be anti-inflammatory and anti-aging effect. EGCG is one of the many components of catechins, but also the highest content of EGCG. But EGCG easily oxidized discoloration, and stored in high temperature or alkaline environment, there is a downward trend in concentration. This study observed EGCG prepared in sodium phosphate dibasic, the concentration decreased significantly, on the other hand, if EGCG prepared in Calcium chloride or EDTA-2Na, stored in 40 degrees high temperature environment, the concentration did not show a significant downward trend. This study used high performance liquid (HPLC) with UV detector (UVD), observed EGCG in different storage environment, or add other substances after the concentration of the trend, and prepared a standard curve by external standard method to quantify the concentration of EGCG.