The mechanism of cell growth inhibition induced by trans-chalcone on human cervical cancer cells.

• Main Authors: HSU, TZU-YEN, 許子彥
• Other Authors: Chih-Jui Chang
• Format: Others
• Language: zh-TW
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/n97te7

碩士 === 慈濟大學 === 分子生物暨人類遺傳學系碩士班 === 105 === In decades, cancer is the one of leading cause of death around the world. Although, there are some strategies to cancer treatment, the side effects of anticancer drug is still a problem to be resolved. Recently, the natural compounds of vegetables, fruits and spices, flavonoids were known to reduce the incidence of cancer. Trans-chalcone, the precursors of flavonoids, also has been demonstrated to decline cancer cell growth, anti-fungal, anti-inflammtory and apoptosis induction. However, the relationship between apoptosis induction and anti-cancer activity is not clear. Here, we found the novel mechanisms of anti-cancer effect of trans-chalcone involved in induction of cancer cell apoptosis. First, the cell viability was reduced by trans-chalcone treatment, and the multipolar spindles were found when cells exposed to trans-chalcone during mitosis. These spindle and centrosome abnormalities resulted in mitotic arrest. Moreover, the activity of aurora A kinase was declined by trans-chalcone treatment in HeLa cells, and cells occurred apoptosis after trans-chalcone treatment. According to the cell viabilities results, the cytotoxicity of trans-chalcone was higher in HeLa cells than that in human lung fibroblast (MRC-5) cells. In conclusion, the activity of aurora A kinase was affected by trans-chalcone, which resulted in multipolar spindle formation. The anti-proliferation effects of trans-chalcone on cancer cells were induced by cell cycle arrest.