| Summary: | 碩士 === 實踐大學 === 食品營養與保健生技學系碩士班 === 105 === Although probiotics are known to enhance the host immune response, their roles in modulating immunosenescence during aging remain unclear. The present study aimed at determining the influence of lactic acid bacteria (LAB) on immunosenescence in SAMP-8 mice. The fifty-six 16 weeks old SAMP-8 mice divided into four groups: comparison group (Non-aging), control group (Aging C), LAB 1X group (108 CFU/day/per mice) and LAB 10X group (109 CFU/day/per mice). For the first study, 16 weeks old mice were analysed with aging appearance evaluation, behavior test and intestinal permeability test. After sacrificied, we collected brain, blood, intestinal fluid and spleen for immune analysis to determining whether SAMP-8 mice could be animal model of immunosenescence. For the secend study, animals were administered LAB for twelve weeks. And then, animals were analysed with aging appearance evaluation, behavior test and intestinal permeability test. After sacrificied, we collected brain, blood, intestinal fluid and spleen for immune analysis to determining whether LAB has the potential to improve immunosenescence. The results of first study showed that phagocytosis and immune response of aging C group were significantly decreased as compared to non-aging group. Serum and gut inflammatory cytokines were increased in aging C group. Intestinal barrier function and gut length were lowered in aging C group. Aging C group was appeared depression. Based on our previous studies that SAMP-8 mice could be animal model of immunosenescence. The results of secend study, appearance evaluation found that feeding LAB has anti-aging in the SAMP-8 mice. In immunity, feeding LAB enhanced phagocytosis and immune response, decreased inflammatory cytokines. LAB supplementation could increase intestinal barrier function, gut length and gut immune response, reduced gut inflammatory cytokines. In addition, feed the bacteria could improve mood disorder. Based on above results, long term lactic acid bacteria supplementation improves the immunosenescence in SAMP-8 mice and it is positively correlated with intestinal function and brain function improvement.
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