| Summary: | 碩士 === 國立虎尾科技大學 === 機械與電腦輔助工程系碩士班 === 105 === The surface modification of biomaterials with better biocompatibility and antibacterial feature has been a challenge for many years. Plasma electrolytic oxidation (PEO) is a rising technology which can produce porous surface for cell adhesion and improves the biocompatibility for further implantation. Tantalum pentoxides (Ta2O5) and zinc oxides (ZnO) have been used as the material of implants due to their good cell biocompatibility and antibacterial property on the microorganisms.In this study, a pre-treatment of surface modification on titanium (Ti) substrates has been made by PEO. Porous oxide layers formed, and Ta-Zn-O films were then deposited onto the porous surface of Ti substrates by using twin-gun high power impulse magnetron sputtering (HIPIMS) and Radio Frequency (RF) sputtering.This study aims to obtain Ta-Zn-O films with different zinc contents by adjusting the zinc target power through the sputtering system. Pure tantalum (Ta), zinc (Zn) and zinc oxide (ZnO) were selected as the target materials. Antibacterial properties and biocompatibilities were analyzed through Staphylococcusaureus(S.a), Aggregatibacteractinomycetemcomitans(A.a), human osteosarcoma cell (MG-63) and Human Skin Fibroblast (SKF).
The surface morphology, crystal structure, and bonding state of the films were identified by scanning electron microscopy (SEM), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS). An in vitro MTT method was used to analyze cell activities, where the influence of cell adhesion morphology and biocompatibility of the specimen on the human skin fibroblast and human osteosarcoma cell(MG-63) were observed. A Syto9 fluorescent staining method was used to observethe survivalrate of Staphylococcus aureus and Aggregatibacteractinomycetemcomitans in the antibacterial tests.
The results showed that the Ta2O5 film had the most hydrophilic effect among thePEO-treated and coated specimens.The MTT cell activity test results showed that the Ta2O5 film had the optimal SKF cell activity.The Ta-Zn-O film containing 4.36 at.% of Zn the best antibacterial property.
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