Biological characteristic of Ta2O5 and ZnO coated titanium pretreated by plasma electrolytic oxidation

• Main Authors: Yi-Jou Lin, 林毅柔
• Other Authors: Yin-Yu Chang
• Format: Others
• Language: zh-TW
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/em8u37

碩士 === 國立虎尾科技大學 === 機械與電腦輔助工程系碩士班 === 105 === In recent years, plasma electrolytic oxidation (PEO) technology has been widely used in biomedical sciences for bones, joints, and dental implants because it can utilize different formulations for preparation of porous titanium surfaces and increase its corrosion resistance.Thisprocess can effectively increase the fusion of implants and the body. This study hopes to use plasma electrolytic oxidation to form a porous titanium oxide (TiO2) surface on pure titanium samples before deposition of antibacterial oxide films. A sputtering technique (a type of physical vapor deposition method) was used to deposit zinc oxide (ZnO) and tantalum pentoxide (Ta2O5) as films on the porous surface that has been processed with PEO. By this process, we study the mechanical properties and aim to achieve better anti-bacterial properties and cellular biocompatibility. This study used potassium phosphate (K3PO4) and potassium hydroxide (KOH) as electrolyte solutions for preparation of the porous titanium oxide (TiO2) surface. High Power Impulse Magnetron Sputtering(HIPIMS) was used for the deposition of tantalum pentoxide (Ta2O5) on the PEO treated titanium surface. Radiofrequency sputtering (RF) was used to deposit zinc oxide films. Staphylococcus aureus, Actinobacillusactinomycetemcomitans, human osteosarcoma cells (MG-63), and human skin fibroblasts (SKF) were used to analyze the anti-bacterial properties and biocompatibility of the samples. 　　This study used X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) and field emission scanning electron microscopy (FE-SEM) to analyze the crystal microstructure and chemical bonds in the film produced. In vitro MTT assays was used to analyze cellular activities, the effects of the test samples on cell adhesion morphology and biocompatibility of SKF and MG-63 cells were observed. The anti-bacterial experiments were carried out using Syto9 fluorescent dyes to measure the bacterial survival rate of Staphylococcus aureus and Actinobacillusactinomycetemcomitans. 　　Results of this study showed that a porous titanium oxide surface was formed after the PEO process which increased the hydrophilicity of the film. The most hydrophilic sampleis the PEO-Ta2O5 samples. Results of MTT cellular activity assays showed that porous titanium oxide surfaces with PEO-Ta2O5 have greater MG-63 cellular biocompatibility compared with films with only tantalum pentoxide (Ta2O5). In addition, anti-bacterial experiments showed that the PEO-ZnOsample have better anti-bacterial properties compared with ZnO test pieces without PEO processed.