Carnosic Acid Inhibits Oral Squamous Cell Carcinoma Cell Proliferation via G2/M Cell Cycle Arrest

• Main Authors: YE, JIN-JIA, 葉晉嘉
• Other Authors: GER, MANG-JYE
• Format: Others
• Language: en_US
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/cgi-bin/gs32/gsweb.cgi/login?o=dnclcdr&s=id=%22105NUK00111001%22.&searchmode=basic

碩士 === 國立高雄大學 === 生物科技研究所 === 105 === Squamous cell carcinoma (SCC) results from the pathological changes of epithelial tissues and squamous cells. The top three cancer types of the head and neck region in Taiwan are oral cavity cancer (>5000 people/year), nasopharyngeal (1000 people/year), and carcinoma laryngeal cancer (500 people/year). Chinese herbal medicines have been known as a potential resource for anti-cancer method. Carnosic acid is a natural phenolic diterpene found in rosemary and common sage. Carnosic acid has diverse biological and pharmacological activities. It has been reported to have several mechanisms to inhibit cancer cells growth. This study is to investigate the mechanisms of oral squamous cell carcinoma SCC-25 and SAS treated with Carnosic acid. MTT assay result shows that oral squamous cell carcinoma cells threated with CA several concentrations the cells viability decrease in a dose-dependent manner. SCC-25 and SAS cells changed the cell morphology at highly CA concentration. Wound-Healing Assay data present that CA could significantly inhibit SCC-25 and SAS migration at highly CA concentration. Flow cytometry results pointed out that CA induced SCC-25 and SAS cells cycle arrest G2/M phase and increase percentage of early apoptosis. We further detect related gene of cell cycle (p53,p27 and p21) and apoptosis (Caspase-3,BAX and PUMA). Real-time PCR data show CA could significantly increase cell cycle-related genes p53, p27, p21 and CyclinB1 transcriptional activity after CA treatment in SCC-25 and SAS cells. Apoptosis-related genes Caspase-3, BAX and PUMA transcriptional activity also increased after CA treatment. Western blot assay shows increment of p53 and p21 expression and cause reducing of cyclin B1 and p-Cdk1 expression after CA series concentration. We suggested CA induced cell cycle arrest in G2/M phase by increased expression of p53, p21, p27 than reduce Cyclin B1 and p-Cdk1 expression in oral Squamous cell carcinoma cells. The results of this study provide information on how to use carnosic acid for oral squamous cell carcinoma. Also, hope to develop a new method to treat patients with oral cancer in the future.