Study on using additive manufacturing technology to fabricate photo-cured PCL- DA+PEG-DA/PGSA scaffold for liver tissue engineering

• Main Authors: Yu-Wei Hsu, 許淯維
• Other Authors: Yih-Lin Cheng
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/3987q3

碩士 === 國立臺灣科技大學 === 機械工程系 === 105 === Currently, the treatment of liver disease is by organ transplantation, but the risk of surgery and the shortage of liver supplement are still the drawbacks that can’t be overcome. Although utilizing tissue engineering can repair the damaging part of liver directly, but there are still some restricts on material and manufacturing process. So is still hard to fabricate a scaffold like a real liver. This study focuses on this two issue that mentions above. We want to develop a biomaterial that combines with both mechanical strength and biocompatibility, and use dye to improve the accuracy of the scaffold to match liver tissue. This study is based on photocurable PCL-DA, and blend with PEG-DA/PGSA to improve the hydrophilic. By tensile test, contact angle test, DSC, and TGA, we check the properties of the material in different concentration. After the material with similar properties was found out, we utilized the DLP-AM system to fabricate the liver scaffold and culture Mouse Liver FL83B Cells on the scaffold to check the cell activities and biocompatibility. The result shows that PCL-DA+PGSA60 1:2 had a better performance in both mechanical strength and biocompatibility and it’s safe to utilize in the human body. In formability, we replaced Phthalocyanine Blue to Sudan Black B for the application in preventing over-curing to fabricate tissue engineering scaffolds. The result shows that in dying concentration 0.04wt% the dimension accuracy can be controlled within 8% and the biocompatibility is good.