Heterostructural nanocrystal for tumor-targeted photodynamic/photothermal therapy

• Main Authors: Si-Han Chen, 陳思翰
• Other Authors: Jia-Yaw Chang
• Format: Others
• Language: zh-TW
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/jw7wq4

碩士 === 國立臺灣科技大學 === 化學工程系 === 105 === In this study, a heterostructure Cu2S-CIS in oil phase via high temperature pyrolysis method were synthesized. The phase transfer was carried out by using hyaluronic acid (HA) and termed as HA-CIS. Further, using EDC/Sulfo-NHS cross-linker HA-CIS was conjugated with a photosensitizer chlorin e6 (Ce6) termed as Ce6-HA-CIS to be applied for photothermal/photodynamic tumour targeted therapy. The synthesis, composition, size, shape and optical properties of the nanomaterial were characterized by using UV-visible absorption and fluorescence spectroscopy, ICP, FT-IR, zeta potential, and HR-TEM. In addition, the Ce6-HA-CIS nanomaterials have excellent light absorption properties in the near-infrared (NIR) region. In vitro and in vivo assessments using mouse melanoma (B16F1) and zebrafish embryos respectively, reveals that Ce6-HA-CIS nanoprobes exhibit low toxicity. The photothermal conversion efficiency of Ce6-HA-CIS nanocomposites was calculated to be up to 32.8%. In vitro B16F1 photothermal and photodynamic results show efficacy of treatments of the cancer cell. The confocal scanning laser imaging shows the nonoprobes effectively internalized in the B16F1 cells. Further, the ablation of cancer cells were confirmed by Trypan blue staining. Plus, human normal cells (HEL) were used to confirm that when the materials were irradiated with lasers for treatment, only tumour cells were destroyed and the normal human cells were not affected. Through, the effect of fluorescence development clearly identify the location of tumour cells and shoot different Z-axis to prove the function of the target to facilitate the follow-up clinical trials.