Amyloid protein nanofibrils membrane prepared from whey protein isolate for adsorption of butterfly pea flower extract and filtration of silver nanoparticles
碩士 === 國立臺灣科技大學 === 化學工程系 === 105 === Amyloids are aggregated proteins that self-assembled into fibrils. The proteins or polypeptides generally form β-sheet structure under a denaturation condition before aggregate into long fiber. β-Lactoglobulin is one of often used proteins for amyloid fibrils ge...
| Main Authors: | , |
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| Other Authors: | |
| Format: | Others |
| Language: | zh-TW |
| Published: |
2017
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| Online Access: | http://ndltd.ncl.edu.tw/handle/4beu75 |
| Summary: | 碩士 === 國立臺灣科技大學 === 化學工程系 === 105 === Amyloids are aggregated proteins that self-assembled into fibrils. The proteins or polypeptides generally form β-sheet structure under a denaturation condition before aggregate into long fiber. β-Lactoglobulin is one of often used proteins for amyloid fibrils generation. Whey protein isolate (WPI) has rich content of β-Lactoglobulin was found in this study can be used directly for the preparation of amyloid fibrils under the condition of pH 2 and heating to 80°C for 20 h. The as-prepared fibrils have an averaged diameter of 15 nm and length of more than 2 μm as observed by AFM. The zeta potential of the fibrils was measured to be +12.9 mV at pH 2 and became zero charge at pH 5.2. The amyloid nanofibrils prepared from WPI could be easily fabricated into membrane by filtering the amyloids suspension using 0.2 μm cellulose acetate microfiltration membrane. Clitoria ternatea (butterfly pea flower) extract that has rich content of anthocyanin was easily concentrated by filtering through the WPI amyloid fibrils membrane. The anthocyanin collected on the membrane exhibited strong antioxidant activity as measured by DPPH assay. The WPI nanofibrils membrane could also work as a nanofiltration membrane for the recovery of silver nanoparticles. Silver nanoparticle (AgNP) prepared by citrate reduction method with particle size about 31 nm could be very effectively collected by the nonofibrils membrane that 96.8 % of AgNP was recovered.
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