Evaluation of silver nanoparticles-incorporating polyurethane-based nonwoven mats for using as antimicrobial dressings on wound management

• Main Authors: Shih-Ting Kao, 高詩婷
• Other Authors: Meng-Yi Bai
• Format: Others
• Language: zh-TW
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/77353518638493737962

碩士 === 國立臺灣科技大學 === 醫學工程研究所 === 105 === The study of antimicrobial Polyurethanecro-based dressings, is reported in here. Polyvinylpyrrolidone (PVP) protected silver nanoparticles were produced by chemical reduction method using ethylene glycol as the reducing agents silver nanoparticles. Fourier transform infrared spectroscopy (FT-IR) and high resolution X-ray photoelectron spectrometer (XPS) confirmed the chemical interaction between the hard segments of PU and silver nanoparticles. Transmission Electron microscopes (TEM) indicated that silver nanoparticles is extrapped in/on the nonwoven mats. The morphology and diameter of Ag/PU nonwoven mats are investigated by high resolution field-emission scanning electron microscope (FE-SEM). The Ag/PU nonwoven mats are composed of electrospun fiber with 175.7~260.5 nm in diameter. The images of FE-SEM revealed the porosity in 50.52~53.61% and pore area in 0.37~0.64 μm2 estimated by DiameterJ created for ImageJ. The qualitative antimicrobial analysis based on the inhibition zone test confirmed the antimicrobial activity and the quantitative analysis based on agar extraction process represented the antimicrobial death rate of 98.26~99.84% against Staphylococcus aureus (BCRC10781). The Ag/PU nonwoven mats showed good permeability and absorption according to moisture vapour transmission rate (MVRT) and water absorbency, respectively. In Ag ion release profile, we found that Ag ion reveals the burst release within 12 hours, showing maximum concentration at 0.69 μg/mL, and releases continuously up to 44 hours. Cytotoxicity assay showed that Ag/PU nonwoven mats reveal no significant cytoxicity toward fibroblast cell. LPS-induced macrophage test showed that Ag/PU nonwoven mats have anti-inflammatory ability. In vivo, full thickness skin removal (1.2×1.2 cm) was performed on the back of C57BL6/J mice in noninfected and infected animal models. Four groups of functional dressings were tested in this work including Aquacel, PU, 0.17M, and 0.34 M. After 10 days of treatment in infected animal models, Aquacel, PU, 0.17 M and 0.34 M were the closure area of the wound falling within 21.48%, 28.51%, 42.12% and 35.02%, respectively. After 10 days of treatment in fected animal models, Aquacel, PU, 0.17 M and 0.34 M were the closure area of the wound falling in infected animal models within 22.05%, 57.08%, 48.75% and 43.72%, respectively. Based on the histological analyses, the 0.17 M treated group outperformed all other groups in wound healing.