| Summary: | 碩士 === 國立臺灣大學 === 口腔生物科學研究所 === 105 === Periodontal bacteria A. actinomycetemcomitans and P. gingivalis were inoculated on commercialy grade 4 pure titanium discs (15 mm in diameter, 2 mm in thickness, and Ra=1.3) respectively to form in vitro biofilm. They were divided into five groups, Group 1 received no treatment and served as control. Group 2 received bacteria inoculation without treatment. Group 3 received bacterial inoculation, curette debridement and PBS irrigation. Group 4 received bacterial inoculation, curette debridement, PBS irrigation and ultrasonication. Group 5 received bacterial inoculation, curette debridement, PBS irrigation and chlorhexidine treatment. SEM result indicated that ultrasonication was noted to eliminate all bacteria on the titanium disc and was thought to be the most effective way to remove bacteria in this study. The result of HEPM cells adhesion assay indicated that attached cells were decreased even bacteria were completely removed by ultrasonication when compared with the titanium disc without bacteria inoculation. We thus coating endotoxin on titanium disc and showed increased in contact angle. However, endotoxin couldn’t be detected using FTIR and XPS. Limulus ameboycte lysate result showed presence of endotoxin after ultrasonication. We concluded that re-osseointegration failure after peri-implantitis is due to endotoxin residues on the implant.
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