CD44 targeted delivery of hyaluronic acid-Doxorubicin/NIR dye conjugates as nanocarrier for the photothermal/chemotherapy

• Main Authors: Wan-Yun Lien, 連婉蘊
• Other Authors: Ming-Jium Shieh
• Format: Others
• Language: en_US
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/9ftgv8

碩士 === 國立臺灣大學 === 醫學工程學研究所 === 105 === In this study, a photothermal drug, IR-780, and a chemotherapy drug, doxorubicin, were conjugated to hyaluronic acid respectively to form two kinds of amphiphilic polymers, HA-IR and HA-DOX. Based on the amphiphilic property, the HA-IR and HA-DOX mixture can self-assembly to nanoparticles, HA-IR/DOX NP. With different HA-IR/HA-DOX ratios, HA-IR/DOX NPs exhibited particle size from 120 to 170 nm with relatively narrow size distribution. The stability test showed that the HA-IR/DOX NP were stable in 4°C storage condition. From in vitro test, HA-IR/DOX NP targeted to CD44-overexpression cancer cell line, MDA-MB-231. The HA-IR/DOX NP showed photothermal effect and phototoxicity both in vitro and in vivo. On the other hand, the fluorescent property of IR-780 provide a function of in vivo imaging, which shows that the HA-IR/DOX NP accumulated to the tumor via EPR effect and CD44-targeting ligand. The antitumor effect showed that the HA-IR/DOX NP combined the chemo-therapy and the photo-thermal therapy.