Surface Functionalization of Gold Surfaces with Polypeptide: A Low-Fouling Zwitterionic Surface for Detect Placenta Growth Factor

• Main Authors: Wei-En Hsu, 許偉恩
• Other Authors: 林啟萬
• Format: Others
• Language: zh-TW
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/ccqrq8

碩士 === 國立臺灣大學 === 醫學工程學研究所 === 105 === In Taiwan, the incidence of colorectal cancer has been increasing year by year. Colorectal cancer is the first occurrence of all cancer. The incidence rate of colorectal cancer has become first for seven consecutive years and the mortality rate also ranked third, only behind liver cancer and lung cancer. According to many cancer studies in the past, placental growth factor(PlGF) is the important marker that can help us to know the potential patient and give them some treatment. In addition to this, PlGF can also be used as one of the risk factors for prognosis recurrence. In this study, I want to know if PlGF exists in patient''s serum. I used surface plasmon resonance(SPR) technology for detection of PlGF. The reasons being that SPR has many advantages, like high sensitivity, real-time detection, non-Fluorescent label and so on. Moreover, I used the antibody as my sensing element. It has a good ability to combine with the target. However, PlGF exists in the serum contains a large amount of protein and will be affected by non-specific adsorption, therefore resulted in the error signal. In order to solve this problem, I used the mixed amino acid sequence for protein anti-fouling and antibody modification in this study. This simple method can achieve protection of surface, and also can be used to detect if PlGF exists at the same time. In the solution, the measurement method proposed in this study has been able to effectively detect the presence of PlGF, and the limit of detection(LOD) is 2pg/ml. Due to the impact of non-specific adsorption, if compare the actual clinical sample with the existing ELISA technology, this study also have sixty precent accuracy.