Role of Caveolin in Stretch-induced Focal Adhesion Dynamics

• Main Authors: Hung-Yu Chang, 張宏宇
• Other Authors: 趙本秀
• Format: Others
• Language: en_US
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/66035782538502458932

碩士 === 國立臺灣大學 === 醫學工程學研究所 === 105 === When exercising, our muscles and ligaments will be subjected to mechanical stimulation. These stimuli can promote cell proliferation, differentiation or even change their behavior. Using bioreactors, we can observe changes in focal adhesion dynamics upon mechanical loading. Caveolae, a membrane microdomain composed of caveolin, may play an important role in the regulation of focal adhesion dynamics. Previous studies have shown that caveolae are involved in the reorganization of focal adhesion during cell migration. On the other hand, the shear stress-induced cytoskeletal rearrangement is also related to caveolin. However, whether the stretch-induced focal adhesion reorganization is also controlled by caveolae has not been explored in detail. In this study, we tried to find out the role of caveolae under mechanical loading by uniaxial stretch and inhibiting the function of caveolae. Results indicated that stretching released Src from caveolae, which affect the size and number of focal adhesions, may be one of the regulators of focal adhesion dynamics upon mechanical loading.