The Growth Inhibitory Effects of Aqueous Leachates from Agricultural Wastes on Chlorella vulgaris

• Main Authors: Yi-Chun Yao, 姚怡君
• Other Authors: Wen-Shang Hou
• Format: Others
• Language: zh-TW
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/p7uq4p

碩士 === 國立臺灣大學 === 生物環境系統工程學研究所 === 105 === Agricultural Wastes due to the sources of scattered, lack of centralized collection and recycling mechanism is not perfect, generally more in the origin of the direct pile, composting or burning, etc., in order to solve the shortage of agricultural waste recycling and decomposition surplus of material problems remaining, enlarging the volume of leaching fallen leaves, rice straw and tea residue with distilled water by the ultraviolet disinfection sterilization (UV-C 237.5 nm), hot air drying and cutting (φ ≦ 10mm), on the process control, the technologic paramenters of leaching temperature, leaching time and dosing ratio. Batch culture Chlorella vulgaris for continuous 96hr, the filtrate after leachingwas filtered with a quantitative filter paper (4 μm), which the concentration, pH and EC value of Chlorella vulgaris were recorded. The regression equation and 50% lethal concentration (LC50) were obtained by GraphPad Prism and SPSS. The results showed that most of the aqueous leachates with strong anti-algae had a significant low pH value, but the pH value of the aqueous leachate from Terminalia catappa fallen leaves was not significantly lower than that of the other materials, and the total rate of inhibition was still higher than the average, may be caused by plant decay release of secondary metabolites; In addition to changes in the pH value of the water, the plant with different immersion conditions degradation process also releases other substances that inhibit or promote the growth of algae. The results showed that the aqueous leachate of the same plant had a significant effect on the growth inhibitory of Chlorella vulgaris, and the effects of leaching temperature, leaching time and dosing rate was significant. The aqueous Leachates from agricultural wastes in the impact of algae growth, is a variety of different factors integrated results. The relationship between the effective algae concentration and the bioavailability of the aqueous leachates was analyzed by the Daphnia magna hydrostatic method. The lethal concentration of the immersion liquid on the Daphnia magna was calculated by using the Daphnia magna as the experimental organisms. Using probit mode was used to fit the dose-response curve to find the virulence regression equation to predict the relevant parameters of the effective algae concentration.