| Summary: | 碩士 === 國立臺灣大學 === 森林環境暨資源學研究所 === 105 === Berberis sect. Wallichianae in Taiwan can be found in subalpine and montane-temperate areas. Most of them are morphologically and ecologically different, only few are sympatry. Previous phylogenetic study (2014: Phytotaxa 184: 61–99) based on three chloroplast DNA sequences region strongly support them, 11 species, as a monophyletic group endemic to Taiwan, but the phylogenetic relationship among them in the radiation are unresolved. Here we sample all of Taiwanese endemic species (i.e., B. aristatoserrulata, B. brevisepala, B. chingshuiensis, B. hayatana, B. kawakamii, B. mingetsensis, B. nantoensis, B. pengii, B. ravenii, B. schaaliae, and B. tarokoensis) and endemic species from Philippines and China, as the out groups. We constructed a well-supported phylogenetic tree based on 32,505 SNPs filtered from RAD-Seq data. The lineage of three group corresponding to geographic and morphological data. Except for the argument between B. kawakmii and B. pengii, the phylogenetic tree confirmed the delimitations of previous species and two new species (B. alpicola and B. morii sp. nov.). With the results of species niche modeling (Last glacial period) and chromosome number (2n=28), we infer to main speciation by geographic isolation. The result also confirms the utility of RAD-Seq data for reconstructing phylogenetic relationships in young and rapid lineages.
|
|---|
