Survey on Brain Tissue 3-D Reconstruction using different Histology techniques

• Main Authors: Po-Hang Tseng, 曾柏杭
• Other Authors: Chen-Yuan Dong
• Format: Others
• Language: en_US
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/46430982128125380387

碩士 === 國立臺灣大學 === 物理學研究所 === 105 === Holoprosencephaly, a kind of brain disease, is usually identified by ultrasonography on fetuses during gestation and confirmed by Magnetic Resonance Imaging (MRI). In the most severe cases the fetus carries craniofacial malformations Cyclopia and Proboscis above the eye with the nose absent. In this research we use the standard histological method to study the microscopic anatomy of brain tissue from human postmortem fetal brain with the Holoprosencephaly disease. Consecutive paraffin slices stained with Hematoxylin and Eosin were imaged by light microscopy and the images were then reconstructed into 3D stacks for more information on the anatomy of the disease. We also study the possibility of using agarose for embedding brain tissue along with the oscillating blade cutting technique to obtain better 3D image from slices with two-photon microscopy.