Fabrication and Characterization of Human Adipose Stem Cell-derived Scaffold via Nanofibers for Enhancing Angiogenesis

• Main Authors: Shing Tak Li, 李承德
• Other Authors: Jiashing Yu
• Format: Others
• Language: en_US
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/90635149392292459080

碩士 === 國立臺灣大學 === 化學工程學研究所 === 105 === Biomedical researchers found great interest in extracellular matrix (ECM) scaffolds derived from cultured cells for tissue engineering applications. ECM scaffolds can be prepared from autologous cells to generate autologous ECM (aECM) scaffolds. It can avoid the undesired host responses that may be induced by allogenic or xenogenic materials and circumvents the limited supply of autologous tissues. In this study, we first fabricate random and align PLGA meshes as a template for cell culturing using PLGA electrospun fibers. Afterward, Human adipose stem cell (hASCs) were seeded onto the PLGA template. Cell-ECM-PLGA constructs were formed by the cultured cells in the PLGA mesh after seven days. Finally, the whole product was decellularized by freeze-thaw cycling and tri-sodium phosphate aqueous solution treatment to remove the undesired PLGA template. The ECM left behind will be freeze-dried and sterilized. Finally, hASCS were seeded onto the ECM scaffold and tested in vivo by using Chick Chorioallantoic Membrane (CAM) assay. The results showed that the ECM scaffold provided wonderful effort in angiogenesis. And the ECM scaffold fabricated in the align PLGA mesh showed a better result compare to the random PLGA meshes.