| Summary: | 碩士 === 國立臺灣海洋大學 === 生命科學暨生物科技學系 === 105 === Gene detection is the one of the important diagnostic tests that can provide useful information to understand the fitness or illness of organs and systems, which enables early detection of serious diseases. The current method for analysis of DNA is through amplification and quantification of DNA by quantitative polymerase chain reaction (qPCR), which has high sensitivity and accuracy. Although qPCR method has these advantages, the high cost of qPCR instrument limits its wide application. In this project, we aim at developing a highly sensitive, high throughput and low cost analysis platform for specific DNA detection by employing noncovalent DNA-modified-gold nanoparticles as probes (probe DNAAu NPs). Considering the disadvantage of the high cost of using thiolated DNA to modify on Au NPs surface, we will use DNA containing poly-adenine (poly-A) tail having high affinity towards Au NPs to prepare probe DNAAu NPs. We believe that employing poly-A tailed DNA can reduce the cost to 20% in comparison with using thiolated DNA probe. The simple and rapid colorimetric assay will be achieved by target DNA-induced aggregation of probe DNAAu NPs when the target DNA is hybridized with probe DNA. As a result, the concentration can be determined by monitoring the absorption at ~520 nm. This method can provide a rapid, sensitive, highly specific and high throughput analysis platform for detecting DNA. These advantages meet the requirements for the clinical diagnosis, and broader application than qPCR method becauseof their low cost and easy handling of instruments. We believe this analysis platform will become a potential tool for DNA detection in commercial clinical diagnosis.
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