| Summary: | 碩士 === 國立臺灣海洋大學 === 生命科學暨生物科技學系 === 105 === Natural enzymes play an important role in many biological systems and they are widely applied in bioanalysis and nano-biotechnology. However, the disadvantages of natural enzymes such as denaturation, sensitiveness to temperature, high cost and complicated preparation methods limit their applications. Thus, the development of artificial enzyme mimetics has been attracting much attention. Recently, the increasing availability of nanoparticles has created widespread interest in their use as enzyme-like nanomaterials because of their large surface-to-volume ratio, high surface energy and stability. In this work, we synthesized a series of poly(thymine) (T60) templated-copper oxide based nanocomposites and studied their oxidase (OX), peroxidase (POX) or catalase (CAT) mimic activities. Among our synthesized copper oxide based nanocomposites, T60-copper oxide-platinum nanocomposites (T60-CuxO/Pt NPs) exhibited distinct oxidase-mimic activity. We demonstrated that inorganic mercury ions (Hg2+) or organic mercury ions such as [methylmercury (MeHg+), ethylmercury (EtHg+) and phenylmercury (PhHg+)] can inhibit the oxidase-mimic activity of T60-CuxO/Pt NPs through specific d10-d10 metallophilic interactions. Hence, T60-CuxO/Pt NPs can be applied as a probe to detect inorganic and organic mercury with a limit of detection (LOD) of 3 nM. To improve the selectivity of the T60-CuxO/Pt NPs probe towards organic mercury, we employed selenium nanoparticles (Se NPs) as a masking agent. We have demonstrated the Se NPs/T60-CuxO/Pt NPs system enables selective detection of MeHg+ in real water samples (in this case, tap water, river water and sea water).
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