| Summary: | 碩士 === 國立臺灣海洋大學 === 食品科學系 === 105 === Blackfoot disease is a vascular disease occurred early in the southwest of Taiwan. The disease is caused by arsenite. In addition, malnutrition is a common feature of patients, especially protein deficiency. On the other hand, blackfoot disease appeared endemic areas with a higher probability of diabetes according to epidemiological studies. Therefore, the toxic effects of arsenite and different casein on diabetic and non-diabetic rats were investigated. In this study, the diabetic and non-diabetic rats in 8 groups daily treated diet with different casein (2% & 20%) and arsenite (500 ppm) or saline by oral administration for 8 weeks. The results showed that arsenite exposure inhibited the activities of catalase and superoxide dismutase, and decreased the glutathione level after 8-weeks feeding. Especially in the feeding of casein-deficiency feed, the enzyme activity and glutathione level showed more lower. On the other hand, diabetic rats showed to decrease the antioxidant and increased insulin resistance value (Homeostasis Model Assessment-Insulin Resistance; HOMA-IR), blood glucose, insulin, triglyceride and total cholesterol concentrations due to pancreatic beta cells damaged. However, in the feeding of casein-deficiency feed, the concentrations of the above indicators are lower. Further, the diabetic rats treated with arsenite exposure and casein-deficiency feed, the tail arterial hyperplasia appeared significantly. Hence, arsenite exposure and casein deficiency could increase the damage in rats, but only arsenite exposure didn’t cause significant damage to diabetic rats.
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