| Summary: | 碩士 === 國立臺灣師範大學 === 化學系 === 105 === Resveratrol (3,5,4'-trihydroxy-trans-stilbene) has been proved to be anti-cancer, anti-inflammatory, cardiovascular protective and is probably able to promote metabolism as well as prolonging life of human beings. In this study, two preparation strategies, including direct dilution and a quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction, were examined for the quantification of resveratrol in red wines. A 1:1 wine/water dilution, 100% acetonitrile as LLE solvent, 300 mg primary and secondary amines (PSA) plus 300 mg anhydrous magnesium sulfate (MgSO4) as dSPE sorbent were found to give the best results. The dilution and optimal QuEChERS method combined with high performance liquid chromatography – mass spectrometry (HPLC-MS/MS) were further validated. A linear range between 5-500 ppb with LOD at 2.5 ppb and LOQ at 5.0 ppb by QuEChERS method are achieved, that shows broader linear range and superior sensitivity in comparison with dilution approach. The recoveries with the two preparation strategies ranged from 97.4% to 111.3% with RSD ≤ 4.9% (n=3). Eventually, resveratrol were detected in 3 out of 7 red wines from local markets, with concentrations within 5.5 to 498 ppb. To the best of our knowledge, this is the first report that combines efficient QuEChERS extraction with LC-MS/MS for the quantification of resveratrol in red wines.
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