Investigation of the properties of adipose-derived stem cells and BALB/c 3T3 fibroblasts grown on silk fabric-based scaffolds

• Main Authors: Lin, Li-Tsen, 林俐岑
• Other Authors: Chang, Hwan-You
• Format: Others
• Language: en_US
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/7arps5

碩士 === 國立清華大學 === 分子醫學研究所 === 105 === A hernia is a common disease occurs when an organ protrudes through a tissue holding it in the normal position. Surgeons use meshes to repair hernias. Most of the meshes are made of synthetic materials such as polyethylene, polypropylene, polyester and polytetrafluoroethylene that may easily lead to foreign body reactions. Silk fibroin from silkworm is a nature polymer applied widely in tissue engineering, possessing excellent mechanical property and biocompatibility. We proposed that silk fibroin has a potential to be used as a hernia repair mesh when knitted into a fabric. This study utilized silk fabric, obtained from a commercial source, as a scaffold to support cell growth. We investigated the characteristics of cells grown on the scaffold and determined whether the scaffold evokes an immune response. First, BALB/c 3T3 and adipose-derived stem cells (ADSCs) cultured on a silk fabric with or without dissolved fibroin or collagen coating were examined by LIVE/DEAD staining. Cell viability of BALB/c 3T3 and ADSCs was measured using alamarBlue assay. Results showed that silk fabric with fibroin coating improved BALB/c 3T3 and ADSCs growth. Furthermore, we found that basic fibroblast growth factor (bFGF) and hepatocyte growth factor (HGF) were produced in a higher level from ADSCs cultured on silk fabric with fibroin coating. Next, we tested TNF and IL-6 production from macrophage line RAW264.7 cultured on silk fabric. The result showed that silk fabric would not trigger TNF and IL-6 production from RAW264.7. Furthermore, silk fabric would not degrade in culture medium for several days. Therefore, silk fabric with fibroin or collagen coating can be developed into a novel hernia repair mesh in the future. To sum up, silk fibroin and collagen provide a suitable matrix for cell growth on silk fabric.