Miniaturization of Molecularly-Imprinted Polymer Beads and Their Applications to Detection of Drug Residue in Foods

• Main Authors: Chong,Shih-Cheng, 鐘士誠
• Other Authors: Hong,Chien-Chong
• Format: Others
• Language: zh-TW
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/83y7x2

碩士 === 國立清華大學 === 動力機械工程學系 === 105 === In this thesis, molecularly-imprinted polymer beads fabricated by emulsion droplet methods to realize the tiny beads and their applications to Detection of Drug Residue in Foods. The study contain simulation, the design of microchannel、the chips are fabricated and characterized. Then, discussion on solution property (dielectric constant and chemical structure). The behavior of droplet formation, such as the omicrochannel width, two-phase flow ratio, surfactant consentration, particulate polymerization, drug adsorption testing such as specificity and selectivity, and particulate package, have been well investigated. The microchannel designs are draw by using AutoCAD .In the experiment, the chips are fabricated by using photolithography process, and then oxygen plasma for bonding. The experimental results show that tiny and homogeneous droplets can be generated, as the microchannel width equal to 50 µm ; droplets decreases as the flow rate of continuous phase increases under the flow conditions of 6 µL/hr for emulsion phase and 30~600 µL/hr for carrier phase, The smallest particle size can reach 15μm. In the droplets polymerization experiment. As the curing energy reaches 60 W / cm2, the micro-droplet can be polymerizated to form microparticles by using ultraviolet light (λ = 365 nm).According to experiental result, the specificity of the developed Ractopamine-imprinted beads is up to 580% and selectivity coefficient equal to 17.07(DC)、3.68(PEN); The specificity of the developed Doxycycline-imprinted beads is up to 415% and selectivity coefficient equal to 6.65(RAC)、2.06(PEN), the adsorption time is 60 seconds. When the total surface area per unit weight of the particle is increased by 8.9 times, the molecular weight of the adsorbed molecule increases by 1.86 times. The microbeads form a column on-chip, when the thickness of the stickers and the volume of the dispersion liquid were 120 μm and 0.5 mL, respectively. The developed droplet chips in this research have the potenials of hight-througput droplets and synthesis the smallest MIP beads.In future, the droplet chips can be fuether synthesis of nanomaterials, such as carbon nanotube.