| Summary: | 碩士 === 國立屏東科技大學 === 生物機電工程系所 === 105 === Biochars(BC) is the product of pyrolysis of biomass in the absence of air. Biochar has many properties such as porosity, high chemical stablization, high water holding capacity, high sorption etc., and is mainly used in soil. In many studies its were comfirmed that biochar can improve physical and chemical properties in soil and increase crops yield. However, biochar was discussed and researched with soil overall change and crops growth, but the mutual relationship of biochar and soil microoganism, like fungi that can improve the nutrient sorption of plant roots, was less studied. Therefore in this study we prepared lemon peel biochar(LPBC) and rice husk biochar(RHBC) and applied two biochars into soild and liquid cultivation of Trametes versicolor mycelium. Based on the data of mycelium coverage, mycelium dry weight, and polysaccharide content, we can study that biochars can improve mycelium growth and yield or not.
The results showed that LPBC and RHBC replaced glucose as carbon source of solid state cultivation was feasible. 2 g LPBC replaced cultivation had the best mycelium coverage with 100% in Day 7. And 2 g RHBC powder replaced cultivation had the best mycelium coverage with 99.5% in Day 7. Those datas are all better than the control, with 99.3% mycelium coverage. In liquid state cultivation, 5 % LPBC additive had the best mycelium dry weight and polysaccharide content with 0.835 g and 0.228 mg/l respectively, and
elevated about 2.9 times and 6.7 times compared to control respectively. In RHBC, 3% RHBC powder additive had the best mycelium dry weight and polysaccharide content with 0.774 g and 0.160 mg/l respectively, and elevated about 2.7 times and 4.7 times compared to cotrol respectively. After these experiment, we find out that LPBC is better than RHBC when uesd in Trametes versicolor mycelium cultivation and the data can help us path the way for the future application in mushroom cultivation bag industries.
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