| Summary: | 碩士 === 國立屏東科技大學 === 生物機電工程系所 === 105 === Banana is one of the important items of fruit nowadays. Banana pseudo-stem is rich with lignocellulose and it is always dumped or burned in the fields, induce the ecological environment pollution. Trametes versicolor, contain a goodness of lignocellulose and which high amounts of the active ingredient with a secondary metabolite. Therefore, this study will demonstrate the feasibility of banana pseudo-stem as alternative carbon sources Trametes versicolor culture fermentation liquid feasibility. In this experiment, organic acid adding induce more versicolor weight of fungus, the amount of Laccase enzyme activity and polysaccharides.
From result, adding banana pseudo-stem will increase fungus dry weight, Laccase activity, and yield of polysaccharides, and organic acids added will speed up the fermentation to gain the best polysaccharides yield. But higher concentration of citric acid and acetic acid will cause poor Laccase activity. The best ratio of banana pseudo-stem is 75%, to obtain the best polysaccharides yield 0.344mg/l.
In conclusion, adding 1g of citric acid will enhance the yield of fungus, and reduce fermentation to provide a new develop.
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