| Summary: | 碩士 === 國立屏東科技大學 === 森林系所 === 105 === Cinnamomum kanehirae is an endemic and valuable species. Because of morphological similarity and hybridization between C. kanehirae and its relates, it is difficult to identify between C. kanehirae and its relates. Therefore, it is more important to develop the molecular tool to identify C. kanehirae species. Microsatellite DNA with neutral and high polymorphism is a suitable tool for conservation of endemic species, and species identification. In this study, the 14 microsatellite loci were developed in C. kanehirae. The polymorphism analysis showed that numbers of alleles were range from 7.000-24.000, observed heterozygosities were range from 0.295-0.660 and polymorphism index content were range from 0.255-0.905. The cumulative PI values of 14 microsatellite loci for C. kanehirae, C. micranthum and C. camphora were 3.79×10-15, 1.4×10-7 and 2.37×10-4, indicating the high potential for individual discrimination of three species. According to the frequency of unique allele for each microsatellite, 9 of 14 microsatellite loci can be effectively used to identifiy C. kanehirae C.micranthum and C. camphora. The individuals of C. kanehirae, C.micranthum and C. camphora were divided into three different clusters in PCoA, STRUCTURE analysis and UPGMA tree. The UPGMA tree showed C. micranthum and C. camphora are very closely related. We also used blind test to determine the species origins of 20 unknow samples, and all unknow samples were assigned to C. kanehirae species. The DUT1-3 sampes has genetic compositions of C. kanehirae and C.micranthum, DUT2-2 has genetic compositions of C. kanehirae and C. camphora based on STRUCTURE analysis, indicating both samples may be hybrids. The microsatellite loci developed in this study could be used to clearly identify C. kanehirae, and its relates and seedling. Futhermore, these microsatellite loci could be applied to assess genetic diversity of C. kanehire for developing conservation strategies.
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