Numerical Simulation and Experimental Investigation of Laser Polymerase Chain Reaction System

• Main Authors: Liu, Jui-kai, 劉睿凱
• Other Authors: Wang, Yao-Nan
• Format: Others
• Language: zh-TW
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/kx8y38

碩士 === 國立屏東科技大學 === 車輛工程系所 === 105 === The polymerase chain reaction (PCR) can directly duplicate the specific DNA sequence through the heating and cooling of thermal cycles, which allows large amount duplication form few DNA. This study using the advantages of high efficiency, high energy concentration and stable output of laser to replace the traditional heating methods. In this study, numerical simulation and verification by the experimental and computational fluid dynamics (CFD) methods. The study integrated mechatronics with Arduino chip for the control system, and argon ion laser with mechanism system for PCR cycling, and a photomultiplier (PMT) with optical system for real-time fluorescence detection of PCR products. In PCR laser cycle, the sample is heated to the standard temperature 95 °C from ambient temperature with heat rate 15.4 °C/sec. In the PCR operation temperature in the range 60 °C to 95 °C with heat rate 10.2 °C/sec, this cooling cycle to 60°C with cooling rate 23.3 °C/sec. Comparing with heat rate and temperature in simulation model and PCR system, the 40 seconds, in heating reaches 102.3 °C, in the numerical simulation result and 95 °C in the experimental result, the deviation is about 7.4 %. In PCR numerical simulation result, it spend 1.5 second to decrease to 56.8 °C, from 102.3 °C in the contrast condition, the experimental result is 60°C, and the deviation is 5.3 %. The result between numerical simulation and experimental result have high consistent, and fluorescent number has amount of increase in PCR system. The laser system can finish 40 PCR cycles within 260 seconds. The system could be used for testing many simples at the same time in the future. Keywords：Integrated mechatronics、Laser PCR、Numerical simulation、 Fluorescence detection