Taxonomic characterization of novel species bacteria isolated from freshwater

• Main Authors: Huang,Cheng-Wen, 黃澄文
• Other Authors: Sheu,Shih-Yi
• Format: Others
• Language: zh-TW
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/07551398649253402704

碩士 === 國立高雄海洋科技大學 === 海洋生物技術研究所 === 105 === In this study, we collected samples from the freshwater environments in Taiwan to research on bacterial diversity and bacterial taxonomic characterization. The potential novel species bacteria were screened and subjected to identification and classification. Freshwater samples were collected from a calla-lily field of Yangming Mountain in Taipei , an amaranth field of Luwild Township in Taitung , a taro field of in Luye Township in Taitung , the Beishi river in Fangliu Township in Pingtung , the farmland irrigation water of East stone Township in Chiayi , a paddy field of East stone Township in Chiayi. There were 20 bacterial strains isolated that containing one Gram-positive bacterium and nineteen Gram-negative bacteria. Phylogenetic analyses based on 16S rRNA gene sequences indicate that these Gram-negative bacteria belong to the class Gammaproteobacteria, class Betaproteobacteria, class Alphaproteobacteria or class Flavobacteria. Among them, strains TPY-10T、Teta-03T、Jyi-02T、Dbr-01T、Npb-02T、Tese-01T、Tese-05T and Tese-07T might be the potential novel species bacteria and strains TPY-10T、Teta-03T、Jyi-02T、Dbr-01T、Npb-02T and Tese-05T were subjected to further identification and classification in this study. Strain Npb-02T was isolated from the water of the Beishi river of Fangliu Township in Pingtung. Cells are Gram negative rod bacteria. Colonies are white colored, circular, round and smooth with entire margins. Phylogenetic analyses based on 16S rRNA gene sequences showed that the closest neighbor strain is Vogesella perlucida DS-28T with the highest sequence similarity 98.35%. On the basis of the genotypic, chemotaxonomic and phenotypic data, strain Npb-02T represents a novel species in the genus Vogesella, of which the name Vogesella amnigena sp. nov. is proposed. The type strain is Npb-02T (=BCRC80887T =LMG28419T). Strain Teta-03T was isolated from the water of a taro field in the Luye Township in Taitung. Cells are Gram negative rod bacteria. Colonies are soil yellow colored, circular, convex round and smooth with entire margins. Phylogenetic analyses based on 16S rRNA gene sequences showed that the closest neighbor strain is Novosphingobium soli CC-TPE-1T with the highest sequence similarity 96.88%. On the basis of the genotypic, chemotaxonomic and phenotypic data, strain Teta-03T represents a novel species in the genus Novosphingobium, of which the name Novosphingobium colocasiae sp. nov. is proposed. The type strain is Teta-03T (=BCRC80538T =LMG27385T =KCTC32255T). Strain Jyi-02T was isolated from the farmland irrigation water of East stone Township in Chiayi. Cells are Gram negative rod bacteria. Colonies are orange yellow colored, circular, convex round and smooth with entire margins. Phylogenetic analyses based on 16S rRNA gene sequences showed that the closest neighbor strain is Novosphingobium Soli CC-TPE-1T with the highest sequence similarity 97.63%. On the basis of the genotypic, chemotaxonomic and phenotypic data, strain Jyi-02T represents a novel species in the genus Novosphingobium, of which the name Novosphingobium arvoryzae sp. nov. is proposed. The type strain is Jyi-02T (=BCRC80537T =KCTC32422T). Strain Dbr-01T was isolated from the water of a paddy field of East stone Township in Chiayi. Cells are Gram negative rod bacteria. Colonies are yellow colored, circular, convex round and smooth with entire margins. Phylogenetic analyses based on 16S rRNA gene sequences showed that the closest neighbor strain is Novosphingobium mathurense SM117T with the highest sequence similarity 97.04%. On the basis of the genotypic, chemotaxonomic and phenotypic data, strain Dbr-01T represents a novel species in the genus Novosphingobium, of which the name Novosphingobium oryzaihumi sp. nov. is proposed. The type strain is Dbr-01T (=BCRC80536T =LMG27387T =KCTC32257T). Strain Tese-05T was isolated from the water of an amaranth field of Luwild Township in Taitung. Cells are Gram negative rod bacteria. Colonies are bright yellow colored, circular, convex round and smooth with entire margins. Phylogenetic analyses based on 16S rRNA gene sequences showed that the closest neighbor strain was Novosphingobium mathurense SM117T with the highest sequence similarity 96.11%. On the basis of the genotypic, chemotaxonomic and phenotypic data, strain Tese-05T represents a novel species in the genus Novosphingobium, of which the name Novosphingobium ipomoeae sp. nov. is proposed. The type strain is Tese-05T (=BCRC80904T =LMG28838T =KCTC42656T). Strain TPY-10T was isolated from the water of a calla-lily field of Yangming Mountain in Taipei. Cells are Gram negative rod bacteria. Colonies are cream white colored, circular, convex round and smooth with entire margins. Phylogenetic analyses based on 16S rRNA gene sequences showed that the closest neighbor strain is Cellvibrio mixtus subsp. mixtus ACM 2601T with the highest sequence similarity 97.75%. On the basis of the genotypic, chemotaxonomic and phenotypic data, strain TPY-10T represents a novel species in the genus Cellvibrio, of which the name Cellvibrio zantedeschiae sp. nov. is proposed. The type strain is TPY-10T (=BCRC80525T =LMG27291T =KCTC32239T).