Long-term Exposure to Nicotine Derivatives and Arecoline Accelerates and Contributes to The Progression of Head and neck Squamous Cell Carcinoma

• Main Authors: HO,CHUN-AN, 何埻安
• Other Authors: NEIH,SHIN
• Format: Others
• Language: zh-TW
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/v6a58q

碩士 === 國防醫學院 === 病理及寄生蟲學研究所 === 105 === Head and neck squamous cell carcinoma(HNSCC) is one of the leading causes of cancer-related death in Taiwan and worldwide. Chewing betel nut, smoking, and alcoholism are the three major risk factors which may contribute to the development of HNSCC. Recent reports have demonstrated that long-term exposure to either nicotine derivatives or arecoline may be associated with tumor progression of HNSCC. 　　In this study, the cell model of long-term exposure to nicotine derivatives and arecoline was established. A comparative analysis using SCC-25 and Fadu cells with or without nicotine derivatives and arecoline exposure was performed to evaluate cell proliferation, migration, and invasion as well as the expression of epithelial-mesenchymal transition (EMT) marker, drug-resistance gene expression, cancer stem cell properties, and anti-apoptotic activity. 　　As a result, either long-term nicotine derivatives or arecoline exposure, or exposures to both risk factors was found to stimulate cell proliferation, enhance migration and invasion, induce EMT with morphological alterations in a dose-dependent manner, increase stemness, and attenuate apoptosis.