Effects of extraction methods and treatments on antioxidant and anti-glycation characteristics of spent coffee ground

• Main Authors: Shih, Yu Chen, 施又甄
• Other Authors: Yang, Huai Wen
• Format: Others
• Language: zh-TW
• Online Access: http://ndltd.ncl.edu.tw/handle/uas5cu

碩士 === 國立嘉義大學 === 食品科學系研究所 === 105 === As coffee culture came into recent vogue, the amount of spent coffee ground (SCG), a major byproduct, has also increased dramatically. Therefore, SCG becomes a substantial waste causing environmental impact regardless the possible retention of bioactive component form coffee bean. The aim of present study was to investigate antioxidant and anti-glycation characteristics of the SCG using different extraction methods (heated water, 60% methanol, 50% ethanol) subsidized by defatting and alkaline pretreatments. Non-dialyzed alkali extract (AEn) of SCG contained the highest amount of total phenol (9.57 mg GAE/g), flavonoids (4.85 mg QE/g), condensed tannins (7.63 mg catechin/g) and caffeic acid (3.96 mg/g); this extract also exhibited profound ABTS•+ scavenging , ferrous ions chelating, reducing power, and ferric reducing antioxidant power activities; whereas, the ethanol extract presented the highest potency of chlorogenic acid (1.01 mg/g) and DPPH radical scavenging activities. The results of present anti-glycation study indicated that AEn-SCG possessed inhibitory effects on maillard reaction and BSA-Fru fructosamine, and suppressed the advanced glycation end products synthesized throughout BSA-Fru, BSA-MGO and Arginine-MGO models. The ethanol extracted counterpart presented the best inhibition rate of dicarbonyl compounds in BSA-Fru model. In conclusion, AEn-SCG possesses the best antioxidant and anti-glycation activities followed by ethanol extract; however, ethanol extraction could still be a better option considering the convenient approach toward product development. The present results indicate that SCG is a natural source subjected to extraction for antioxidant and glycation inhibitor with respect to the application of diabetes relieving meanwhile increase the added-value environmental waste.