Screening and Evaluation of Yeast Antagonists for Biological Control of Botrytis cinerea

碩士 === 國立彰化師範大學 === 生物學系 === 105 === Gray mold (Botrytis cinerea) is one of the most important diseases of strawberries (Fragaria × ananassa Duchesne) worldwide. Although there are chemical fungicides using for controlling the growth of B. cinerea, the risk of the fungus to develop chemical resistan...

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Bibliographic Details
Main Authors: Chen,Pei-Hua, 陳珮華
Other Authors: Chou,Jui-Yu
Format: Others
Language:en_US
Published: 2017
Online Access:http://ndltd.ncl.edu.tw/handle/2acv7x
Description
Summary:碩士 === 國立彰化師範大學 === 生物學系 === 105 === Gray mold (Botrytis cinerea) is one of the most important diseases of strawberries (Fragaria × ananassa Duchesne) worldwide. Although there are chemical fungicides using for controlling the growth of B. cinerea, the risk of the fungus to develop chemical resistance and the demand of consumers to reduce using chemical fungicides drive us to find an alternative method to control the fungus. There are various naturally occurring microbes that aggressively attack on plant pathogens and benefit plants by disease suppression and hence referred to as biocontrol agents. Among the variety of biological control agents available for use, screening of potent biocontrol agents is necessary for their further development and commercialization. In this study, 24 strains of yeast with antagonistic ability against gray mold were isolated and the antifungal activity of the volatile and diffusible metabolites was evaluated. Putative mechanisms of action associated with the biocontrol capacity of yeast strains against B. cinerea were studied by means of in vitro and in vivo assays. The volatile organic compounds produced by the JYC1146 strain could be useful in the biological control of plant pathogens and therefore emerge as a possible alternative to fungicides with lower environmental impact.