Piezo-Ring-on-Chip Microfluidics Device for Mass Spectrometry Interfacing

• Main Authors: I-Chao Lei, 雷以兆
• Other Authors: Chia-Wen Tsao
• Format: Others
• Language: en_US
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/29750986205161029697

碩士 === 國立中央大學 === 機械工程學系 === 105 === The interfacing methods for microfluidics to mass spectrometry should be compatible for the microfluidic chips that are already in use. An external interfacing device can be easily applied to the microfluidic chips without changing the original designs on the chips. In this study, we achieved the spray from chip by introducing a ring-shaped piezoelectric acoustic atomizer (piezo-ring), which is commercially available and low-cost, and used it as the microfluidic to MALDI – MS interfacing device. We designed a holder pattern at the outlet of the PDMS chip, making the chip be able to mount the piezo-ring and directly spray samples from the outlet without any intermedium such as capillaries. The sample spreading issue on the fluidic outlet surface was discussed and the pulsatile spraying behavior of piezo-ring was observed as well. We also added the visualize particles into the microchannel for the further evaluation of pumping effects to the microchannel by piezo-ring actuation. The deposition process of matrix was obtained before the MS analysis. The MS signals of peptides were then demonstrated by mass spectrometry and we compared the signal intensity of peptides by piezo-ring with traditional pipetting method. The low use of sample volume and uniform signal intensity were achieved by the piezo-ring-on-chip device.