| Summary: | 碩士 === 國立中央大學 === 化學工程與材料工程學系 === 105 === Human pluripotent stem cells (hPSC) of human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) have the potential ability to differentiate into many kind of cell types originated from three germ layers: endoderm, mesoderm and ectoderm cells such as dopamine-secreting cells for Alzheimer disease treatment and insulin-secreting cells for diabetes treatment.1 However, it is a challenging issue to guide hPSCs to differentiate into our desired lineages of cells due to their variety of differentiation ability. The fate of differentiation of stem cells is determined by different factors existed in the microenvironment of hPSCs: bioactive molecules, cell-cell interactions, physical factors and cell-biomaterial interaction. It is a reasonable strategy to mimic the stem cell microenvironment for the differentiation of hPSCs into specific lineages of cells using optimal biomaterials for hPSC culture. Currently, it has not yet investigated which extracellular matrices (ECMs) or nanosegments derived from ECMs promote hPSCs differentiation into cardiomyocytes. We developed nanosegment-grafted biomaterials having different elasticity for hPSCs differentiation into cardiomyocytes.
We developed several biomaterials for hPSCs differentiation into cardiomyocytes. We prepared (1) ECM-coated dishes where ECMs are Matrigel, Synthemax II, fibronectin (CellStartTM), laminin-521, laminin-511, recombinant vitronectin, vitronectin, and fibronectin. On day 0, we replaced the expansion medium into cardiomyocytes differentiation medium containing the GSK3B inhibitor. On days 1-2, we observed that 30%~40% of the cells were died and detached from the surface. However, the center of the colony of living cells were getting thicker and became compact. The cells were differentiated into cardiomyocytes between days 5-6. On day 8-10, we successively observed the contracting colonies on the surface.
We evaluated the optimal ECM-coating dishes for differentiation of hPSCs into cardiomyocytes. The results for cardiomyocyte induction from hPSCs will be used in clinical application and in the investigation of molecular mechanism of specification and maturation of cardiomyocytes.
|
|---|
