Chondrogenesis of Stem Cells in Functional Peptide Materials

• Main Authors: Lai, Tsung-Sheng, 賴宗聖
• Other Authors: Lin, Hsin-Chieh
• Format: Others
• Language: zh-TW
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/k2664u

碩士 === 國立交通大學 === 生醫工程研究所 === 105 === Regenerative medicine is becoming more popular in tissue repair. Several researches have been focusing on combining stem cell and biomaterials for tissue regenerations. Materials ranges from polymer scaffold to even small molecular weight hydrogel all have been to have some tissue engineering applications. In this study, we focused on the ability of self-assembling peptides, and hypothesized that they can promote cell chondrogenesis and osteogenesis. We implemented a series of experiments and analysis to confirm our hypothesis. Firstly, we designed five different functional sequences derived from N-cadherin, and investigated their capability in inducing chondrogenesis after exposing them to a mesenchymal stem cell (MSC) line The levels of chondrogensis were determined by Alcian blue staining and qPCR analysis after culturing the cells with the peptides at a 2D environment for 7、14、21 days. The data showed that either HAVD or HAVDI treated cells expressed more chondrocyte specific genes such as Collagen type II and Collagen type X compared to negative control. However, when the cells were exposed to either HAVD or HAVDI in 3D hydrogel, lower expressions of chondrocyte specific genes were detected compared to HAVS treated cells. Furthermore, the expression of chondrocyte specific gene could increase by modulating the peptide-based material stiffness , in which HAVS containing hydrogel showed the best capability in inducing chondrogenesis after stiffness modulation.