Reverse Microemulsion-Assisted Spherical Reduced Graphene Oxide Composite Synthesis for Photodynamic Therapeutic Application

• Main Authors: Nien, Cheng-Yung, 粘正雍
• Other Authors: Hsu, Hsin-Yun
• Format: Others
• Language: en_US
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/27298919928283816606

碩士 === 國立交通大學 === 應用化學系碩博士班 === 105 === The graphene oxide (GO), has created huge breakthrough development in diverse fields. It was also assessed for drug delivery systems and employed as new formulation for cancer theranostics. Although it has been reported that extended conjugated aromatic ring of GO enabled the absorption of large amount of small molecules for drug delivery vehicles, the size and geometry control was challenging and the agglomeration occurred frequently. Owing to its anisomerous dimensions, the uptake efficiency of cells varies and was not optimized. Herein, we developed a method to enable self-assembly of reduced graphene oxide nanospheres (rGON) under mild condition while the pristine properties of GO were preserved and cellular uptake could be enhanced. Additionally, we employed the methylene blue, a photosensitizer, which could generate singlet oxygen (1O2) in the rGON for photodynamic treatment of cancer cells. Comparing with conventional 2D graphene oxide, 3 folds higher cellular uptake (30 % vs. 10 %) enabled low power density (2 mW) and short irradiation time (1.5 min) to double photocytotoxicity of cancer cells (60 % vs. 30 %), indicating great potential of rGON composites to serve as new photodynamic therapeutics in the future.