Bovine Serum Albumin Detection Based on Blue Phase Liquid Crystals

• Main Authors: Chang, Chung-Huan, 張崇煥
• Other Authors: Lee, Wei
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/80258086805836943448

碩士 === 國立交通大學 === 光電系統研究所 === 105 === In this study, it is the first time that blue phase liquid crystals are used for biomedical detection. Because of the Bragg reflection caused by the blue phase liquid crystals, we can observe the change of the blue phase reflection peak from different BSA concentration sample and realize the quantification in liquid crystal biomedical detection. In the experiment, there are two different mixture being investigated and the effects of detection from each mixture are also different. In the first part result of the E7 doped with chiral R5011, the uniform blue phase structure with DMOAP alignment showed sharp Bragg reflection peak and the presence of BSA disturbed the alignment of blue phase liquid crystal, causing the multi-domain lattice structure. In the result, two reflection peak appeared in the spectrum while the BSA concentration was higher than 106 g/mL, or there was only one peak on spectrum and the intensity of the only peak increased as the BSA concentration decreased. The other result was from mixture of E7 doped with S811 and the Bragg reflection peak blue shifted as the BSA concentration increased only if the concentration was lower than 106 g/mL. When the BSA concentration was higher than 106 g/mL, the strong scattering happened induced by the disorder lattice. Thus we used the spectrum of smectic A phase which was appeared at lower temperature in the same mixture to assist in detecting the BSA concentration while the concentration is higher than 106 g/mL.