Label-Free Detection of Lung Cancer miRNA Bio-marker With Polysilicon Nanowire Field-Effect Transistor

• Main Authors: Zheng, Jian-Ming, 鄭建銘
• Other Authors: Yang, Yuh-Shyong
• Format: Others
• Language: en_US
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/07522871692136624235

碩士 === 國立交通大學 === 生物科技學系 === 105 === Prognosis of lung cancer at its early stages is vital for improving the survival rate of patients. MiRNAs are short non-coding ribonucleic acid molecules that play key roles in a class of genes involved in human tumorigenesis. Current methods for the detection of miRNA are facing different challenges and problems such as time-consuming, low-sensitivity and requirement for labeling. Polysilicon silicon nanowire field-effect transistor (pSNWFET) has great potential to be developed to a powerful biosensor because of its capability for highly sensitivity, real-time and label-free detection of nucleic acid. In this research, functionalized pSNWFET has been demonstrated to achieve specific and ultrasensitive detection of tumor-derived miRNAs, let-7a and miR-21, which may serve as biomarkers for lung cancer. Optimal conditions for specific target-probe hybridization on the nanowire surface were determined. Finally, miR-21 in A549 lung cancer cells extract was determined using pSNWFET and its sensitivity was found comparable to the standard qPCR method at femtomolar regime. We demonstrated in this study that a platform for the screening of miRNA associated with lung cancer in clinical serum sample can be achieved through a pSNWFET based miRNA biosensor. We expect that this platform can be an excellent choice for future application in biomedical and prognosis of lung cancer.