Lactobacillus plantarum TWK10 strain supplementation for health and exercise performance improvement in mice: Gut microbiota and liver proteome perspectives

• Main Authors: Yi-Ming Chen, 陳奕鳴
• Other Authors: Chi-Chang Huang
• Format: Others
• Language: zh-TW
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/61299717000841593163

博士 === 國立體育大學 === 運動科學研究所 === 105 === Propose: Lactobacillus plantarum (L. plantarum) is a well-known probiotic among the ingested-microorganism probiotics (i.e., ingested microorganisms associated with beneficial effects for the host). However, few studies have examined the effects of L. plantarum TWK10 (LP10) supplementation on exercise performance, physical fatigue, and gut microbial profile. Methods: Male Institute of Cancer Research (ICR) strain mice were divided into three groups (n= 8 per group) for oral administration of LP10 for six weeks at 0, 2.05 × 108, or 1.03 × 109 colony-forming units/kg/day, designated the vehicle, LP10-1X and LP10-5X groups, respectively. In our study, we evaluated LP10 effect on the exercise performance, change of muscle type, gut microbiota metagenomic analysis and perform quantitative LC-MS/MS-based proteomic profiling of livers to provide corroborative evidence for differential regulation of molecular and cellular functions affecting metabolic processes. Results: LP10 significantly decreased final body weight (p<0.05) and increased relative muscle weight (%) (p<0.05). LP10 supplementation dose-dependently increased grip strength (p < 0.0001) and endurance swimming time (p < 0.001) and decreased levels of serum lactate (p < 0.0001), ammonia (p < 0.0001), creatine kinase (p = 0.0118), and glucose (p = 0.0151) after acute exercise challenge. The number of type I fibers (slow muscle) in gastrocnemius muscle significantly increased with LP10 treatment. In addition, serum levels of albumin, blood urea nitrogen, creatinine, and triacylglycerol significantly decreased with LP10 treatment. The intestinal microbiota revealed relative abundance of LP10 treatment, Bacteroidetes plylum decreased (p=0.011) and Firmicutes plylum increased (p=0.0093). In liver proteomics analysis, very long-chain acyl-CoA synthetase, peroxisomal acyl-coenzyme A oxidase, microsomal triglyceride transfer protein large subunit, protein disulfide-isomerase were significantly increase by LP10 supplementation (p<0.05). Conclusion: Long-term supplementation with LP10 may increase muscle mass, enhance energy harvesting, and have health-promotion, performance-improvement, and anti-fatigue effects. The mechanism of LP10 improve exercise performance and muscle mass was liver released very-low-density lipoprotein triglyceride (VLDL TG) formed by very long chain fatty acid secretion to provide energy and the VLDL TG was uptake in muscle tissue for energy ultlization. In microbiota opinion, LP10 could transformed the intestinal microbiota to the short chain fatty acid (SCFA) producing bacteria. Above all, LP10 could enhance energy producing by fatty acid oxidation and harvesting SCFA from intestinal.