| Summary: | 碩士 === 國立成功大學 === 細胞生物與解剖學研究所 === 105 === Stem cells can differentiate to endothelial lineage as a source for tissue engineering of vessels. However, the rarity of cell source and low differentiation efficiency are the main limitation for stem cell therapy in vessel regeneration. Our previously study demonstrated a successful endothelial cell (EC) differentiation by integration of biomechanical force and chemical factors, but the mechanism is still unknown. Furthermore, we build up a cell therapy of brain hypoxic-ischemia (HI) model by using human umbilical vein endothelial cells (HUVECs). The differentiated cells will apply to this animal model in the future. By static HUVECs transplantation in brain HI model, brain injury and cell death were decreased. Laminar shear stress (LSS)-treated HUVECs group in animal model had not significantly repair function than static HUVECs. Previously research indicates that CXCR4, a chemoattractant receptor, is reduced by LSS. One paper showed that erythromycin (EM) increased CXCR4 expression. Therefore, we combined LSS with EM to stimulate HUVECs. We found that EM treatment after LSS stimulation of HUVEC transplantation was useful for rescuing brain injury. In this study, we built up successful cell therapy for brain injury model. Furthermore, repair function of ECs can be enlarged by increasing EM-induced CXCR4 expression.
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