Fӧrster Resonance Energy Transfer in the Heterogeneous Assembly of Janus particles

• Main Authors: Shiang-NingYang, 楊翔甯
• Other Authors: Chang-Shu Kuo
• Format: Others
• Language: zh-TW
• Published: 2018
• Online Access: http://ndltd.ncl.edu.tw/handle/7rgz2u

碩士 === 國立成功大學 === 材料科學及工程學系 === 105 === Asymmetric submicron Janus particles were fabricated and co-assembled with 100 nm polystyrene (PS) particles by surface electrostatic force interaction. The ideal co-assembled structure was a tetramer where four 500 nm Janus particles assembled as tetrahedron arrangement with one 100 nm PS particle in the center. The co-assembled structure with different morphologies were mainly determined by the sizes and the ratios of two particles. The co-assembled structure also included the different morphologies. The particle co-assembly was carried out by Janus particles cored with 500 nm silica particles and functionalized with amino-silane in one of their hemispheric surfaces, followed by their conjugation with Atlantic Blue dye or NBDHA. And, the second particles with smaller diameters in the co-assembly were commercially-available PS particles, which were 100 nm polystyrene (PS) particles with carboxylated surface. Two fluorescent dyes, Atlantic Blue and NBDHA, were employed as the FRET donor and acceptor, respectively. Since the FRET took place only when the distance between the donor and acceptor objects was very close, the successful FRET signals indicated the cluster formation from the particle co-assembly. Well-defined particle co-assembly simultaneously initiated the cluster diameter increase as shown in the DLS measurement, as well as the FRET emission from their photoluminescence outputs. Observed the fluorescence color of combination type of co-assembled clusters by confocal microscope to make sure the co-assembled clusters at least included one donor and one acceptor JPs to trigger the FRET effect. Steady particle co-assembly and the related FRET responses realized the sensor mechanism and platform.