| Summary: | 碩士 === 國立成功大學 === 生物醫學工程學系 === 105 === Although we have successfully fabricated poly(glycerol sebacate) (PGS) fibrous membrane by coaxial electrospinning, it remains PGS prepolymer leak-out issue during the fabrication process. In this study, we tended to reduce the ratio of PEO to PLA in the shell solution or use more intensively crosslinked pPGS. On the other hand, one of the advantages of using fibrous scaffolds in tissue engineering is that cell alignment and hence tissue microstructure may be manipulated by the mechanism of contact guidance. Therefore, we focused on fabricating highly aligned PGS fibrous membrane. The core-shell fiber structure was confirmed by TEM. From the SEM images, we successfully mitigated pPGS leak-out from the inner core. Both of PEO and PLA were removed from the PGS fibrous membrane, which were verified by FTIR and DSC. The alignment index of PGS fibrous membrane was 2.06±0.21 indicating that PGS fibers were highly aligned. From the results of cyclic stretching test, we further affirmed that PLA was removed. PGS fibrous membrane also showed anisotropic mechanical properties examining by uniaxial tensile test. It’s Young’s modulus was 0.52-0.98MPa which was similar to soft tissues. Anisotropic PGS electrospun fibers promoted human umbilical smooth muscle cell guidance. We discovered that the degradation rate of PGS fibrous membrane was slower than PGS solid sheet, indicating that the crosslinking degree of PGS fibrous membrane might be higher than PGS solid sheet. The in vitro cytocompatibility of the electrospun membranes was confirmed. The aligned PGS fibrous membrane has the potential to be used for soft tissue engineering applications
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