| Summary: | 碩士 === 國立成功大學 === 生命科學系 === 105 === Asthma is a worldwide disease with symptoms as airway hypercontraction, wheezing and chronic cough and is featured with chronic airway inflammation and obstruction. Increasing prevalence rate of asthma in children is observed in many countries. Allergic asthma is the most popular disease among those asthmatic patients. Patients are over-reacts to foreign allergen such as dust mite or pollen then subjected to asthma attack. House dust mite (HDM) is the most seen allergen. The main source is from Dermatophagoids pteronyssinus (Der p). Xiao-Qing-Long-Tang (XQLT) is a used traditional Chinese medical which treat symptom such as wheezing, cough and sputum. XOTL is proved to be an inhibitory agent against allergic inflammation and wheezing in several allergens, including Der p, induced acute or chronic allergic asthma animal models. It is found oral-fed with XQLT can down regulate toll-like receptor 4 (TLR4) expression in lungs of a Der p induced allergic mice model. In vitro study shows that inhibitory effect of XQLT on TLR4 will be reversed by recombinant soluble TLR4 (sTLR4). It seems that XQLT may exert inhibitory effect on allergic reaction by affecting TLR4 pathway. This project is designed to confirm the inhibitory effect of XQLT on Der p induced TLR4 signal and to isolate possible effector of XQLT by using sTLR4. In this research, XQLT is found to decrease the membranous TRIF-related adaptor molecule (TRAM) which binds to TLR4 specifically in a Der p stimulated cell line. The result will be subsequently applied to isolate TLR4-regulating effector of XQLT.
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