| Summary: | 碩士 === 國立中興大學 === 獸醫病理生物學研究所 === 105 === Clostridium perfringens is a gram-positive anaerobe which is able to form spore. It is widespread in the environment such as soil, sewage, food and feces. It is not only the normal intestinal flora of humans and animals but also zoonosis. Permitting classification of C. perfringens isolates into five toxinotypes based on the production of four typing toxins; that is alpha (CPA), beta (CPB), epsilon (ETX) and iota (ITX).
The aim of this study was to investigate the prevalence, toxinotype, other toxins, Vero cell cytotoxicity and antimicrobial resistance of C. perfringens isolates from different species such as healthy humans, patients, dogs, cats, pigs, chickens, cows and horses in Taiwan.
We collected 1189 samples from different animals (including healthy humans, patients, dogs, cats, chickens, pigs, cows, and horses from Apr. 2015 to Apr. 2017. The C. perfringens isolation rate were 95/225 (42%) in healthy humans, 58/359 (16%) in patients, 52/65 (80%) in dogs, 26/31 (84%) in cats, 113/192 (59%) in pigs, 18/215 (8%) in chickens, 51/76 (67%) in cows, and 6/26 (23%) in horses. Most of these isolates were toxinotype type A and four of them had cpe gene. Two isolates were toxinotype type E and were isolated from human and dog. Three isolates from two cows and one horse were negative for the eight toxigenic genes.
The result of Vero cell cytotoxicity testing revealed that most of isolates did not cause Vero cell cytotoxicity. However, in some isolates, whether with or without cpe gene, could cause significant cell rounding. All isolates are susceptible to vancomycin. Most of isolates are susceptible to ampicillin、metronidazole、cefotaxime. About 25 % isolates are resistance to clindamycin.
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