Role of Platelet on Tumor Progression in Tumor-bearing Mice

• Main Authors: Chiao-Chen Ko, 柯喬偵
• Other Authors: Wen-Ying Chen
• Format: Others
• Language: zh-TW
• Published: 2017
• Online Access: http://ndltd.ncl.edu.tw/handle/41438850311644244268

碩士 === 國立中興大學 === 獸醫學系暨研究所 === 105 === Cancer is currently the leading cause of death in developed countries and second in developing countries. Several studies have shown an significantly increased risk for pancreatic, prostate, breast, colon, endometrial, liver, kidney, esophagus, gastric, gallbladder, and lung cancers in obese adults. A variety of abnormalities in platelet function have been identified in obese individuals, including increased activation and reduced sensitivity to physiological and pharmacological antiaggregating agents. Tumor cells can induce platelet activation through various mechanisms and their metastatic potential correlates with their ability to activate platelets. Since patients with obesity or metabolic syndrome show abnormality in platelets, the role of platelets in obesity-associated malignancy still remain unclear. The purpose of this study is to investigate the relationship between obesity, platelet and malignancy. Male diet-induced obese and matched lean control C57BL/6NCrlBltw mice were inoculated subcutaneously with 1×104 Lewis lung carcinoma (LLC1) cells for 3 weeks to verify the relationship between platelet and obesity-associated tumor. Ectopic implanted LLC1 cells grew and developed to tumor mass and the tumor weight was significantly higher in obese mice than the lean control (P<0.05). Results of ELISA revealed that obesity was associated with increased plasma content of IL-6, thrombopoietin, thromboxane B2 and P-selectin in tumor-bearing mice (P<0.05). Plasma level of P-selectin, a marker for procoagulant activity, was relatively higher in obese tumor-bearing mice than lean tumor-bearing mice. Moreover, there was an increased P-selectin and thromboxane A2 receptor protein expression in tumor tissues removed from obese mice (P<0.05). Taken together, these results suggest that platelets may play a role on obesity and tumor growth, demonstrating that there is a synergistic effect between platelet activity and obesity-associated tumor progression.